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Plasmids
| Plasmids | Relevant characteristics | Source or ref. |
|---|---|---|
| pT7FH-G | ApR; ori pBR322; Φ10P-bglG | 6 |
| pMBP-BglG | ApR; ori pBR322; tacP-malE-bglG | A. Wright |
| pLAX185 | ApR; ori pBR322; lppP-lacP-rpoA | 22 |
| pMKSe2 | ApR; ori pBR322; lacP-rpoB | 23 |
| pT7β′ | ApR; ori pBR322; Φ10P-rpoC | 24 |
| pMKA201 | ApR; ori pBR322; lacP-Φ10P-rpoC | 25 |
In plasmid pMKA201, only the Plac promoter was used for the expression of rpoC because of the lack of T7 polymerase in the host strain. ApR, ampicillin resistance.
