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. 1999 Apr 13;96(8):4348–4353. doi: 10.1073/pnas.96.8.4348

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Copyright © 1999, The National Academy of Sciences

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(A) Schematic representation of the chimeric Cyt c exchange (pHM124/126) and linker-anchor exchange (pHM125/127) cytochromes along the native Cyt c_y^Rs and Cyt c_y^Rc. Control region, Anchor/Linker, and Cytochrome c refer to the various subdomains of these cytochromes. The restriction sites used to create these chimeras are indicated. (B) Immunoblot analysis of various R. capsulatus strains carrying chimeric Cyt c_y-FLAG derivatives with anti-FLAG M2 antibody. Chromatophores were prepared from cells grown in enriched MPYE medium under either Ps (lanes 1–3) or Res (lanes 4–6) growth conditions, and 25 μg membrane proteins were loaded per lane. Lane 1, pHM126/M6G-G4/S4 (Cyt c exchange); lane 2 and 5, pHM127/M6G-G4/S4 (linker-anchor exchange); lane 3, pHM119/M6G-G4/S4 (Cyt c_y^Rs-FLAG); lane 4, pHM126/SL3 (Cyt c exchange); and lane 6, pHM119/SL3 (Cyt c_y^Rs-FLAG).