Table 1.
Bacterial strains and plasmids
| Strain | Relevant characteristics | Refs.178 |
|---|---|---|
| R. capsulatus | ||
| MT1131† | crtD121 RifR, wild-type | 13 |
| Y262 | Overproducer of gene transfer agent | 18 |
| FJ2 | crtD121 Δ(cycARc∷kan) Δ (cycYRc∷spe); cyt c2Rc−cyRc−,Ps− | 3 |
| M6G-G4/S4 | crtD121 qox-260 Δ(cycA∷kan); Qox− cyt c2−,Ps+ | 14 |
| R. sphaeroides | ||
| Ga† | crt, wild-type | 10 |
| Gadc2 | crt, Δ(cycARs∷spe); cyt c2Rs−,Ps− | 19 |
| Gadcy | crt, cycYRs∷kan; cyt cyRs−,Ps+ | |
| Gadc2cy | crt, cycYRs∷kan Δ (cycARs∷spe); cyt c2Rs−, cyt cyRs−,Ps− | |
| Plasmids | ||
| pRK2013 | KanR, helper plasmid | 17 |
| pRK415 | TetR broad host-range plasmid | 17 |
| pSUP202 | AmpR, CmR, TetR, suicide plasmid | 16 |
| pBSII | pBluescript II KS(+), AmpR | 11 |
| pFJ66 | Δ(cycY∷spe); SpeR, TetR | 3 |
| pKD2 | cycYRs on a 1.25-kb EcoRI/BlgII fragment of pUI8180 in pBSII | |
| pHM4 | cyt MA-c2 on a 1.2-kb BamHI/HindIII fragment in pBSII | 11 |
| pHM6 | cycYRc-FLAG on a 1.2-kb BamHI/HindIII fragment in pBSII | 11 |
| pHM8 | cyt MA-c2 on a 1.2-kb KpnI/BamHI fragment in pRK415 | 11 |
| pHM13 | cycYRs on a 2.0-kb XbaI/HindIII fragment in pRK415 | |
| pHM14 | cycARc on a 1.25-kb BamHI/HindIII fragment in pRK415 | |
| pHM15 | cycYRs on a 1.6-kb EcoRI/XbaII fragment of pU1942 (see ref. 9) in pBSII | |
| pHM16 | cycYRs∷kan on a 2.9-kb EcoRI fragment in pBSII | |
| pHM17 | cycYRs∷kan on a 2.9-kb EcoRI fragment in pSUP202 | |
| pHM100 | Like pHM126 but without a FLAG epitope | |
| pHM110 | Like pHM6 but contains an NdeI site at position 87 | |
| pHM112 | Like pHM6 but contains an NheI site at position 371 | |
| pHM113 | Like pHM6 but contains both an NdeI and an NheI site at positions 87 and 371, respectively | |
| pHM118 | Like pKD2 but contains cycYRs-FLAG | |
| pHM119 | pHM118 at HindIII site of pRK415 | |
| pHM121 | cycYRs68∷cycYRc95-FLAG on a 1.2-kb KpnI/XbaI fragment in pBSII (“anchor-linker exchange chimera”) | |
| pHM123 | pHM121 at HindIII site of pRK415 | |
| pHM124 | cycYRc108∷cycYRs82-FLAG on a 1.2-kb KpnI/XbaI fragment in pBSII (“cyt c exchange chimera”) | |
| pHM125 | Like pHM121 but does not contain an NdeI site | |
| pHM126 | pHM124 at HindIII site of pRK415 | |
| pHM127 | pHM125 at HindIII site of pRK415 |
178
If not otherwise indicated, all strains and plasmids were obtained during this work.
†
The R. capsulatus strain MT1131 (RifR crtD) and R. sphaeroides strain Ga (crt) are referred to as wild-type strains, because they are wild-type in respect to their Cyt c profile and growth properties.