Table 3.
Respiratory electron transport activities of various R. capsulatus strains measured by using isolated membrane fragments
| Substrates, concentration | Moles of O2 consumed per h per mg of protein
|
||
|---|---|---|---|
| pRK415/M6G-G4/S4 (Cyt cyRc) | pHM13/SL3 (Cyt cyRs) | pHM8/SL3 (Cyt MA-c2Rc) | |
| NADH, 0.5 mM | 9.6 | 2.2 | 15.2 |
| Rotenone, 5 μM | 0.4 | 0.4 | 0.3 |
| Myxothiazol, 2 μM | 0.4 | 0.3 | 0.2 |
| Antimycin A, 2 μM | 1.7 | 0.5 | 1.8 |
| Cyanide, 50 μM | 0.2 | 0.1 | 0.2 |
| NADH + Cyt c, 50 μM | 19.2 | 19.0 | 21.4 |
Numbers are the mean value of two sets of independent experiments. Cyt MA-c2Rc, membrane-bound derivative of Cyt c2.