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Northern blot of total RNA from wild-type cells, CCL16-B1, and the two mutant cell lines described here, CCL16-B2 and V79-G4. Equal loading of the gels was verified by both staining and labeling of ribosomal RNAs. From molecular mass markers run in an adjoining lane, the size of the mRNA was estimated to be ≈400 nt. The CCL16-B2 mRNA is slightly smaller and reduced in amount (see Results).
