Figure 4.

Comparison of rotenone-sensitive NADH-quinone oxidoreductase activities in wild-type (W), mutant CCL16-B2 (M), and a complemented mutant clone transfected with the NDUFA1-cDNA expression vector (C). Determinations were made by measuring oxygen consumption (respiration) that was stimulated by malate plus glutamate and inhibited by rotenone in cells that had been made permeable with digitonin (see Materials and Methods). Bars represent the average of five such measurements, and the standard deviation is indicated.