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Immunoblotting analysis of several peripheral membrane proteins of complex I in wild-type (W), mutant CCL16-B2 (M), and transfected (complemented) cells (C). A preparation of bovine heart mitochondrial membranes (B) also is included for comparison. Mitochondrial membranes were prepared as described in Material and Methods. Gels were loaded to obtain comparable signals for the β-subunit of the F1-ATPase.
