Table 2.

Fluorescence maxima of MBP-R-PE apo-subunit fusion proteins after attachment of PEB in vivo. Proteins were isolated from E. coli cells containing MBP-R-PE apo-subunit fusions after incubation of cells with PEB. Spectra of fusion proteins were recorded in maltose elution buffer by a fluorescence spectrometer (λ_exc = 530 nm, λ_em = 610 nm). From these values and referenced literature it was concluded that attachment of PEB to cells containing MBP-R-PE apo-subunit fusion proteins yields holo-subunit fusion proteins containing PEB as the sole chromophore.

Excitation fluorescence maximum (nm)	577.5
Emission fluorescence maximum (nm)	584.0
Phycobilin present in fusion protein	PEB