Table 3.
Excitation and emission fluorescence maxima of PEB, and MBP-R-PE apo-subunit fusion proteins after attachment of PEB in vitro. Spectra of free PEB and fusion proteins were recorded in maltose elution buffer by a fluorescence spectrometer (λexc = 530 nm, λem = 620 nm). From values of fluorescence maxima and shoulders (sh), and referenced literature, it was concluded that attachment of PEB to MBP-R-PE apo-subunit fusion proteins in vitro yields holo-subunits containing PEB.
| Phycobilin | Free PEB | Bound and Free PEB |
|---|---|---|
| Excitation fluorescence maximum (nm) | 528.0 | 576.0, 525.0 (sh), |
| Emisssion fluorescence maximum (nm) | 579.0, 625.0 (sh) | 586.5, 625.0 (sh) |