Average fluorescence intensities of cells containing holo-subunits and control cells measured by flow cytometry. Fluorescence intensities of 10,000 cells were analyzed. All cells were incubated with PEB under the same conditions, but only αind and βind cells expressed apo-alpha and apo-beta subunits of R-PE, respectively. These cells had, on average, several times higher fluorescence compared to cells without expressed apo-subunits (αcon, βcon, BL21(DE3)).