Table 2.
Bacterial challenge (CFU) | 106 | 107 | 108 | ||
Time | 36 hrs post infection | 18 hrs post infection | 36 hrs post infection | 18 hrs post infection | 36 hrs post infection |
Control group (non-immunized) | 0 | 1.08 ± 0.14 × 106 | 8.98 ± 1.94 × 106 | 1.11 ± 0.20 × 108 | 2N/A |
PBS | 0 | 4.92 ± 0.94 × 105 | 1.54 ± 0.94 × 106 | 3.16 ± 1.94 × 106 | 2N/A |
IFA | 0 | 2.35 ± 1.12 × 105 | 1.23 ± 1.14 × 106 | 1.80 ± 0.22 × 106 | 2.16 ± 1.10 × 106 |
PHIS-01 | 0 | 3.64 ± 1.44 × 105 | 1.13 ± 2.31 × 106 | 8.90 ± 3.20 × 105 | 5.60 ± 0.94 × 105 |
PHYTOL | 0 | 4.0 ± 0.25 × 103 | 0 | 8.90 ± 1.12 × 104 | 0 |
1 Peritoneal fluid samples from all groups of mice were diluted in LB medium (1:200), and 10 μl of diluted samples were streaked on agar plates using calibrated loops. CFUs were scored 18 and 36 hours following bacterial challenge and recorded as CFU/ml. The results represent the average of two experiments (n = 4 for each group) ± SEM. 2 N/A: Not assessable since all mice were dead within 18 hrs.