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. 1999 Apr 13;96(8):4366–4371. doi: 10.1073/pnas.96.8.4366

Figure 2.

Figure 2

Competition between WT and Δ93–102/NPNG apoLp-III for binding to PL-C-treated LDL. LDL (250 μg of protein) was incubated with 880 mU of PL-C in the presence of WT (250 μg), Δ93–102/NPNG (250 μg), or both WT and Δ93–102/NPNG apoLp-III (250 μg of each) at 37°C. After 40 min, unbound apoLp-III was separated from LDL-bound apoLp-III. The LDL-containing (lanes 1, 2, and 3), and the bottom fractions (lanes 6, 7, and 8) were analyzed by SDS/PAGE. The lanes contained PL-C-treated LDL plus the following: WT apoLp-III (lanes 1 and 6); Δ93–102/NPNG apoLp-III (lanes 2 and 7); and WT and Δ93–102/NPNG apoLp-III (lanes 3 and 8). Standard WT and Δ93–102/NPNG apoLp-III were in lanes 4 and 5, respectively.