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. Author manuscript; available in PMC: 2007 Aug 25.
Published in final edited form as: J Biol Chem. 2006 Jun 28;281(34):24979–24990. doi: 10.1074/jbc.M604589200

Figure 9.

Figure 9

When co-expressed with Orai1, EYFP-Stim1 is not detected at the cell surface by confocal microscopy. HEK293 cells transfected with EYFP-Stim1 and Orai1 cDNA were incubated in PBS with 1.8 mM Ca2+ (top and bottom panels), or in Ca2+-free PBS containing 2 μM thapsigargin for 15 minutes prior to fixation and permeabilization with triton X-100 (bottom panels only). Cells were stained with anti-EYFP Alexa 647 conjugated antibody and analyzed by laser scanning confocal microscopy to detect EYFP and/or Alexa 647.