Figure 4.

Expression of wt and mt p53 in transfected (10)1 cells. (10)1 cells were transfected with wt or mt p53 expression plasmid DNA (1.0 μg) without any 15-LO promoter vector. The cells were grown and lysates were prepared as described in Fig. 3. Western blotting was conducted as described in Materials and Methods. Lanes: 1, (10)1; 2, wt p53; 3, p53–175 (mt p53); and 4, Escherichia coli-expressed p53 protein (standard).