Figure 5.

Expression of mt p53 reduces 12/15-LO protein. (10)1 and (10)1val cells were plated and incubated at 37°C for 24 h before being shifted to either 39 or 32°C for another 24 h. Nuclear and cytoplasmic proteins were extracted for Western blotting as described in Materials and Methods. The 75-kDa protein band reactive with 12-LO antiserum is indicated. (10)1val cells have predominantly wt p53 at 32°C but have mutant p53 at 39°C. (10)1 cells contain no p53 protein.