Figure 1.
RNA polymerase switch variants. (A) An unstable rrn9Δ haploid segregant (NOY684) derived from RRN9/rrn9Δ diploid strain NOY678. A heterogeneous cell population recovered from a tiny rrn9Δ colony formed on a tetrad-dissection plate was suspended in H2O, spread on a YEPD plate, and incubated at 30°C for 7 days. (B) An isolated larger colony (left) and an isolated small colony (right) from the plate shown in A were restreaked on YEPD and incubated at 30°C for 7 days. (C) Non-PSW strain NOY703 and PSW strain NOY878 derived from NOY703 were streaked on YEP-galactose (Gal) and YEPD (Glu) plates and incubated at 30°C for 5 days.