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Loss of heterozygosity study at D3S3522 polymorphic marker in neuroblastic tumors. PCR products were run in a 15% polyacrylamide gel and visualized after ethidium bromide staining (0.5 μg/ml) T: DNA from tumor tissue; N: DNA from peripheral blood of the same patient; M1/M2: 10 bp DNA ladder/1 Kb Plus DNA ladder (Invitrogen™ Life and Technologies, Carlsbad, CA, USA). Sample number 64 showed microsatellite instability.
