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. 2006 Oct 25;6:254. doi: 10.1186/1471-2407-6-254

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Copyright © 2006 Lázcoz et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Promoter methylation analysis of RASSF1A (A), NORE1A (B), BLU (C) and CASP8 (D) in neuroblastic tumors (B, C and D) and cell lines (A), determined by MSP and visualized in 2.5% agarose gels stained with 0.1 μg/ml ethidium bromide. U/M: unmethylated/methylated; M: 1 Kb Plus DNA Ladder (Invitrogen™ Life and Technologies, Carlsbad, CA, USA); B: DNA obtained from blood of a normal donor; IMD (in vitro methylated DNA): positive control, CpGenome™ Universal Methylated DNA (Chemicon International, Inc., Temecula, CA, USA);-: water (no DNA negative control). A) 2: SH-SY5Y; 3: SK-N-SH; 4: SK-N-MC; 5: IMR-32; 6: MHH-NB-11; 7: SK-N-Be(2); 8: SK-N-DZ; 9: BE(2)C. B), C), and D) Numbers on top of each well correspond to DNA from different neuroblastic tumors analyzed.