Table 2.
Primers and conditions for MSPs.
| Gene | Primer forward (5'–3') | Primer reverse (5'–3') | Size (bp) | T (°C) | Cycles |
| RASSF1A | |||||
| - U | GAGAGTGTGTTTAGTTTTGTTTTTG | CCCATACTTCACTAACTTTAAACAC | 183 | 62 | 38 |
| - M | GAGAGCGCGTTTAGTTTCGTTTTC | ACCCGTACTTCGCTAACTTTAAACG | 184 | 58 | 35 |
| NORE1A | |||||
| - U | ATTTATATTTGTGTAGATGTTGTTTGGTAT | ACTTTAACAACAACAACTTTAACAACTACA | 215 | 54 | 40 |
| - M | CGTCGTTTGGTACGGATTTTATTTTTTTCGGTTC | GACAACTTTAACAACGACGACTTTAACGACTACG | 202 | 62 | 40 |
| BLU | |||||
| - U | TTTGTGGGTTATAGTTTGAGAAAGTG | AACAAATTAACCACACCTACAC | 156 | 60 | 30 |
| - M | TTCGTGGGTTATAGTTCGAGAAAGCG | AACGAATTAACCGCGCCTACGC | 156 | 66 | 30 |
| CASP8 | |||||
| - U | TAGGGGATTTGGAGATTGTGA | CCATATATCTACATTCAAAACAA | 320 | 53 | 40 |
| - M | TAGGGGATTCGGAGATTGCGA | CGTATATCTACATTCGAAACGA | 321 | 50 | 40 |
U: unmethylated; M: methylated.