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. Author manuscript; available in PMC: 2006 Nov 6.
Published in final edited form as: Ann N Y Acad Sci. 2006 Apr;1068:297–308. doi: 10.1196/annals.1346.057

FIGURE 1.

FIGURE 1

The osteoclastogenic effect of AZT in RAW264.7 cells (A) RAW264.7 cells were treated with AZT at indicated concentrations in the presence of RANKL (50 ng/mL) for 3 days. Osteoclasts were measured by TRAP staining assay. The Y-axis represents the TRAP intensity per well. (B) RAW264.7 cells were treated with RANKL (50 ng/mL, R) or RANKL plus AZT (25 μM, R + A) for 1–3 days as indicated and total mRNAs were isolated. RT-PCR for tartrate-resistant acid phosphatase (TRAP), calcitonin receptor (CTR), RANK, and GAPDH2 genes was performed.