Fig. 5.

Renal glomeruli from hMSC-treated diabetic mice on day 32. Sections (5-μm) are magnified ×400. (A–D) Glomeruli labeled with antibodies for human nuclei antigen and mouse/human fibronectin. Some human cells that are colabeled have rounded morphology of mesangial cells. (E--H) Glomeruli labeled with antibodies for human nuclei antigen and mouse/human podocalyxin. No colabeling is detected. (J–L) Three-dimensional views of cells in glomeruli labeled for human nuclei antigen and for mouse/human endothelial cells (CD31). Some human cells appear to be colabeled and have the elongated morphology of endothelial cells. As indicated in the three-dimensional views, the CD31 epitope is present on several aspects of the human cells. Arrows, human cells; dotted arrows, planes for deconvolution; dotted lines, outlines of glomeruli. For additional three-dimensional deconvolved images, see Figs. 7–9 and Movie 1, which are published as supporting information on the PNAS web site.