Table 1.
Engraftment assayed by real-time PCR for Alu
| Animal/cells | Days | Pancreas | Kidney |
|---|---|---|---|
| 1/hMSC | 17 | 2.95 ± 0.06 | 6.70 ± 0.06 |
| 2/hMSC | 32 | 1.02 ± 0.31 | 0.05 ± 0.004 |
| 3/hMSC | 32 | 0.78 ± 0.05 | 11.58 ± 2.16 |
| 4/hMSC | 32 | 0.22 ± 0.03 | 0.03 ± 0.05 |
| 5/hMSC | 32 | 0.07 ± 0.01 | 10.62 ± 0.715 |
| 6/hMSC | 32 | 0.04 ± 0.02 | 9.82 ± 1.23 |
| 7/hMSC | 32 | 0.36 ± 0.02 | NA |
| 8/hMSC | 32 | 0.19 ± 0.09 | NA |
| 9/hMSC | 32 | 0.11 ± 0.01 | NA |
| 10–13/hMSC | 32 | ND | ND |
| 14–18/hFibro | 22 | ND | ND |
Values are percentage of human DNA infused as cells. NA, not assayed; ND, not detected; hFibro, human skin fibroblasts. Tissues were assayed either 17 or 32 days (Scheme 1) or 22 days after hFibro.