Fig. 1.

WTAP siRNA-treated cells display growth inhibition in G₂/M phase. (A) The specificity of anti-WTAP polyclonal antibody. COS7 cells transiently transfected with a pcDNA3.1(−)-WTAP-myc-His or empty vector were subjected to Western blot analysis by using anti-WTAP or -myc antibody. Arrows indicate the band corresponding to WTAP (≈50 kDa), detected by an anti-myc antibody. (B) Efficient reduction of WTAP protein was confirmed by Western blots in WTAP siRNA-transfected HUVEC. HUVEC were transfected with WTAP siRNA or control siRNA. Total proteins were extracted 72 h after transfection. (C) Cell growth rates in WTAP siRNA or control siRNA-treated HUVEC. Cell numbers were determined by using a hemacytometer. (D) Cell-cycle analysis was carried out by using siRNA-treated HUVEC. Forty-eight hours after siRNA transfection, cells were harvested and stained with propidium iodide and then analyzed for DNA content with a FACScalibur. ∗, P < 0.05; ∗∗, P < 0.001 vs. contol.