Fig. 4.

WTAP-null mice exhibited proliferative failure, with death resulting at approximately E6.5. (A) Generation of WTAP-null mice. Retroviral integration and disruption of the WTAP gene. VICTR 48 integrated in the intron between exons 1 and 2. NEO, a functional fusion between the β-gal and neomycin resistance genes; pA, polyadenylation sequence; SV40tpA, SV40 triple polyadenylation sequence; PGK, phosphoglycerate kinase-1 promoter; BTK, Burton's tyrosine kinase. (B) PCR genotyping of embryos from WTAP+/− intercrosses by using three primers, A, B, and LTR, to simultaneously amplify both WT (341 bp) and mutated (mut, 255 bp) alleles. ∗, Nonspecific band.