Fig. 5.

Morphology of embryos from WTAP+/− heterozygous matings. (A and B) Heterozygous WTAP+/− embryo at E8.5 (A) and homozygous WTAP−/− littermate (B). Homozygous WTAP−/− embryos are small and present no embryo body. (C–E) Representative H&E stainings of E6.5 normal (C) and abnormal (D and E) embryos cross-sectioned. (E) Some abnormal embryos have a dilated proamniotic canal. (F–I) Representative H&E and WTAP staining of E6.5 embryos sagittally sectioned . (F) Normal embryos exhibit defined layers of cells from embryonic ectoderm and endoderm. (H) Abnormal embryos are smaller and present no defined layers. No specific WTAP stainings were observed in morphologically abnormal embryos (I, compare with G), confirming that these abnormal embryos are homozygous null mutants. (Scale bars: A and B, 500 μm; C–E, 200 μm; F and H, 100 μm.)