Fig. 6.
Cell-cycle-dependent expression of WTAP. (A) Western blots of WTAP and cyclin A2 of the G1, S, or G2/M phase. For synchronization studies, HUVEC were serum-starved for 36 h, then released by medium exchange. The cell-cycle profile was monitored by FACS analysis, and proteins were collected at each time point. (B) Relative protein expression of WTAP was normalized to β-actin expression. (C) EMSA using cyclin A2 mRNA 3′UTR full and the indicated concentrations of either GST or GST-WTAP. Arrowheads indicate the RNA–protein complexes.