Table 1.

Diagnostic classification of reference reader results used to establish accepted composite diagnosis and identify unqualified readers

Level of Diagnosis	Reported Diagnosis	Variable	Composite Diagnosis	Misdiagnosis Demerit Points
Primary	Plasmodia positive or negative	Dichotomous	Agreement >80%	10: false positive 5: false negative
Secondary	Species	Discontinuous (Pf, Pv, Pm, Po)*	Agreement >80% for mono-infection Agreement >50% for mixed infection**	3: infection misclassified
Tertiary	Parasite Count	Continuous	Median count	1: parasite density outside 99% CI

* Pf = Plasmodium falciparum, Pv = P. vivax, Pm = P. malariae, Po = P. ovale, includes any combination of these species

** or less than 50% with PCR confirmation