Table 2.

Giemsa smear results among 24 remaining reference readers for 35 donations subjected to external validation

Donor	Composite Diagnosis	Reader Agreement %	PCR Result	Reference Reader Results	Median Parasite Density (per ul)
12	Pf/Pv	13	PfPv	3Pf/Pv, 21Pf	31,222
8	Pm	50	Pm	12Pm, 2Pm/Pv, 2Pv, 2Pf, 5neg, 1 no sp	95
16	Pm	58	Pm	14Pm, 10Pf/Pm	3,183
6	Pf/Pv	63	PfPv	15Pf/Pv, 6Pf, 3Pf/Po	15,084
11	Pf/Pv	67	PfPv	16 Pf/Pv, 6Pv, 1Pf/Po, 1Pf/Pm/Pv	129,437
50	Pv	83	Pv	20Pv, 3Po, 1Po/Pv	14,895
2	Pv	83	Pv	20Pv, 4Pf/Pv	15,000
15	Pv	88	Pv	21Pv, 2Pm, 1neg	64
17	Pv	88	Pv	21 Pv, 2 Po, 1Po/Pv	1,335
49	Pv	88	Pv	21 Pv, 2 Po, 1Pm/Po/Pv	328
4	NEG	92	neg	22neg, 2 Pf	n/a
52	NEG	92	neg	22neg, 1 Pf, 1Pv	n/a
10	Pf	96	Pf	23Pf, 1Pf/Pv	1,620
20	Pf	96	Pf	23Pf, 1 no species	202
21	Pf	96	Pf	23Pf, 1Pf/Pm	165,500
22	NEG	96	neg	23neg,1Pf	n/a
1	Pf	100	Pf	24Pf	15,453
3	Pf	100	Pf	24Pf	3,542
7	Pf	100	Pf	24Pf	3,669
9	Pf	100	Pf	24Pf	340
13	Pf	100	Pf	24Pf	1,048
18	Pf	100	Pf	24Pf	301,996
19	Pf	100	Pf	24Pf	1,404
23	NEG	100	neg	24neg	n/a
44	NEG	100	neg	24neg	n/a
46	Not used*	50	Pf	12Pf, 12 neg	9
58	Not used*	58	Pv	14Pv, 4Po, 2Pm, 1Pm/Po/Pv, 1Pf/Po, 1neg, 1no sp	63
14	Not used*	63	Pv	15Pv, 4Po, 3Pf/Pv, 1Pm/Pv, 1Pm/Po/Pv	5,191
25	Not used*	67	Pf	16Pv, 2Po, 1Pf, 5neg	48
59	Not used*	71	Pv	17Pv, 1Po, 2Pf, 2neg, 1Pf/Po/Pv, 1Pf/Pm/Po	135
60	Not used*	75	Pf	18Pf, 1Po, 3neg, 2no sp	90
54	Not used*	79	Pf	19Pf, 2Pv, 1neg, 2no species	78
55	Pf* not used	83	Pf	20Pf, 1Pv, 1Po, 1Pf/Pm, 1 no species	129
5	Pf* not used	100	Pf	24Pf	2,625
24	Pf* not used	100	Pf	24Pf	10,885

Pf = Plasmodium falciparum, Pv = P. vivax, Pm = P. malariae

* Donation not included in the U.S. National Institutes of Health Malaria Research and Reference Reagent Resource Center (MR4) malaria slide repository either because composite diagnosis could not be determined or because slides not required for sets