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. 2006 Sep 1;4:16. doi: 10.1186/1477-5956-4-16

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Copyright © 2006 Jin et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of monocyte phosphoproteome by ProQ staining. (A) Specificity of pro Q stain for phosphorylated proteins. Lane 1: Pro Q staining of monocyte lysates. Lane 2: Same cell lysates treated with protein phosphatase (80 units/ml) for 60 minutes at 30°C prior to SDS-PAGE/Pro Q staining. Lane 3: Sypro Ruby fluorescence staining of Lane 1. Lane 4: Sypro Ruby fluorescence staining of Lane 2. A protein band at about 25 kDa is consistent with M_rof protein phosphatase added to the sample. (B) A 2D gel of monocytes stained with Pro Q diamond phosphoprotein dye. Arrows point to the protein spots with IDs and positive for phosphorylation stain.