Figure 3.
Induction of tumor specific-cytotoxic effector responses. Spleen cells were obtained from C57BL/6 mice on day 7 after 3rd immunization with OVA in test adjuvants. BALB/c mice were given injection of test adjuvants 5 days before challenge with the B-cell lymphoma, 2C3 and sacrificed on day 8. Splenocytes harvested were stimulated with either killed E.G7-OVA or 2C3 cells in vitro for 5 days. Effector cells harvested on the fifth day were assayed in a 51Cr-release cytotoxicity assay as described under Methods. The results shown represent the mean of triplicates ± SD from two separate experiments (n = 3 mice per group/experiment). A. OVA-specific CTL response from C57BL/6 mice. B. Tumor-specific CTL response from BALB/c mice