Table 3.

The results of parameter optimization for scenario B (estimation of pseudo-association rate coefficient in a FRAP experiment).

Estimate $K_a^{*}$
Initial guesses			Optimized values
Df (μm2 s-1)	$K_a^{*}$ (s-1)	Kd (s-1)	Df (μm2 s-1)	$K_a^{*}$ (s-1)	Kd (s-1)	RMSE	R2
30	3.00	0.1108	30	30.0032 (30.3523)	0.1108	0.00 (0.01)	1.000 (0.998)
30	1 × 10-3	0.1108	30	29.9982 (30.2455)	0.1108	0.00 (0.01)	1.000 (0.998)
30	1 × 10-6	0.1108	30	30.0031 (30.2468)	0.1108	0.00 (0.01)	1.000 (0.998)
30	1 × 106	0.1108	30	30.0030 (30.2478)	0.1108	0.00 (0.01)	1.000 (0.998)
30	1 × 103	0.1108	30	30.0031 (30.2507)	0.1108	0.00 (0.01)	1.000 (0.998)
30	300.00	0.1108	30	30.0030 (30.3188)	0.1108	0.00 (0.01)	1.000 (0.998)
30	10.00	0.1108	30	30.0030 (30.2115)	0.1108	0.00 (0.01)	1.000 (0.998)
30	0.050	0.1108	30	30.0030 (30.1655)	0.1108	0.00 (0.01)	1.000 (0.998)

The values in parentheses were obtained using corrupted data.