Table 1.
Summary of de novo assemblies for several organisms
Genome | Genome size, Mb | Enzyme | Average genomic fragments size, kb | No. of optical maps | Over-sampling | Overlap calculation, h | Layout-consensus calculation, h | Refinement calculation, h | Overlap yield | Contig yield (mass, Mb) | % of genome covered | Contigs aligned to ref. (mass, Mb) |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Y. pestis | 4.6 | XhoI | 17.3 | 251 | 49× | 0.1 | 0.01 | 0.1 | 691 | 1 (4.8) | 100 | 1 (4.8) |
E. coli | 4.6 | XhoI | 26 | 6,750 | 708× | 31 | 1 | 1 | 184,522 | 1 (4.6) | 100 | 1 (4.6) |
T. pseudonana | 34.5 | NheI | 9.8 | 34,460 | 435× | 2,400 | 1 | 6 | 254,978 | 46 (38) | 100 | 18 (6.4) |
O. sativa | 430 | NheI | 11.8 | 260 × 103 | 268× | 96 × 103 | 1.2 | 1 | 8 × 106 | 307 (236) | 52 | 288 (225) |
Human CHM | 3,200 | SwaI | 13.5 | 213 × 103 | 30× | 57 × 103 | 1 | 2 | 12 × 106 | 219 (150) | 4.6 | 171 (109) |