Fig. 5.

Western blot analysis of PK-treated brain homogenates from diseased wild-type hamsters. (A) Western blot analysis of brain homogenates from animals that developed clinical TSE symptoms after i.c. inoculation with the infectious starting material (start), PrPres generated by sPMCA, and the initial reaction mixture after 2.5¹⁴-fold dilution without PMCA (dilut.), respectively, before (unstabilized) and after (NC-adsorbed) binding to NC particles. (B) Secondary passage from affected animals inoculated with the unstabilized starting material (start) and the sPMCA-derived PrPres (sPMCA) before (unstabilized) and after (NC-adsorbed) adsorption to NC particles, respectively. The brain samples of the examined animals contained large amounts of proteinase-resistant PrP identical to that of the 263K strain used to seed the PMCA reaction in regard to electrophoretic mobility and glycoform pattern. Positions of the molecular mass markers are given on the right (in kilodaltons).