Fig. 2.

Cdk1 can phosphorylate AR Ser-81 and enhance AR protein expression. (A) Predictions for kinases targeting Ser/Thr/Pro sites. NTD, N-terminal domain; DBD, DNA-binding domain; LBD, ligand-binding domain. (B–E) 293T cells were transfected with 100 ng of AR plasmid, together with other plasmids (100 ng each or 0.04–0.2 μg as indicated), with empty pCDNA3.1 vector to equalize total plasmids. After overnight transfection, the cells were incubated in 5% CSS medium with 10 nM DHT for 24 hr, and equal amounts of protein were immunoblotted.