Fig. 6.

Roscovitine inhibits hypersensitive responses to DHT in androgen-independent C4-2 cells. (A and B) LNCaP cells or C4-2 cells were split in RPMI medium 1640 plus 5% CSS for 2 days, and different doses of DHT were then added for 24 hr as indicated. (A) Total RNA was isolated for real-time RT-PCR analysis of PSA expression versus 18S rRNA and levels were normalized to untreated cells. (B) Total proteins were harvested in 2% SDS and equal amounts were immunoblotted. (C) C4-2 cells were split in RPMI medium 1640 plus 5% CSS for 2 days, and different doses of DHT were added for 24 hr, with or without 10 μM roscovitine, as indicated.