Table 1.
Antimicrobial MICs of the ultrashort lipopeptides (μM) and their hemolytic activity
| Peptide designation |
E.c. |
P.a. 27853 | S.a. 6538P | A.b. 19606* | A.fu. 26430 | A.fl. 9643 | C.a. 10231 | C.n. MYA-422 | Hemolysis, % |
||
|---|---|---|---|---|---|---|---|---|---|---|---|
| D21 | 25922 | At 20 μM | At 50 μM | ||||||||
| C16-KLLK | 100 | 100 | 100 | 3.12 | 50 | 6.25 | 12.5 | 6.25 | 1.5 | 9 | 45 |
| C14-KLLK | 25 | 25 | 12.5 | 3.12 | >100 | 6.25 | 12.5 | 6.25 | 2.3 | 13 | 50 |
| C12-KLLK | 50 | 50 | 25 | 12.5 | >100 | 25 | 100 | 12.5 | 3 | 0 | 0 |
| C16-KAAK | 6.25 | 6.25 | 12.5 | 6.25 | 12.5 | 6.25 | 100 | 12.5 | 3 | 0 | 3 |
| C14-KAAK | 50 | 50 | 50 | 12.5 | 100 | 25 | 100 | 50 | 6.25 | 0 | 0 |
| C12-KAAK | >100 | 100 | >100 | 100 | >100 | >100 | >100 | >100 | 50 | 0 | 0 |
| C16-KGGK | 3 | 3 | 6.25 | 6.25 | 12.5 | 12.5 | 12.5 | 12.5 | 1.5 | 8 | 75 |
| C16-KKKK | 6.25 | 6.25 | 3 | 6.25 | 12.5 | 25 | 100 | 25 | 1.5 | 0 | 6 |
| C16-KKEK | 12.5 | 12.5 | 50 | 12.5 | 37 | 25 | 100 | 100 | 6.25 | 0 | 0 |
| C16-GIGK | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | 100 | 6 | 10 |
| Gentamicin | 3.12 | 6.25 | 1.56 | 6.25 | >100 | — | — | — | — | — | — |
| Ampotericin B | — | — | — | — | — | 1.6 | 3.2 | 1.5 | 1.5 | — | — |
The abbreviations used for genus and species names are as follows: E.c., Escherichia coli; P.a., Pseudomonas aeruginosa; S.a., Staphylococcus aureus II; A.b., Acinetobacter baumannii; A.fu., Aspergillus fumigatus; A.fl., Aspergillus flavus; C.a., Candida albicans; C.n., Cryptococcus neoformans. The numbers that follow the abbreviated genus and species names correspond to American Type Culture Collection (ATCC) numbers. Amino acids in italics are the d-enantiomers.
*Gentamicin-resistant.