Fig. 5.

Differential interaction of Tic32 with NADPH, calmodulin, and Tic110. (A) Influence of NADPH on Tic32-calmodulin interaction. Inner envelope vesicles were solubilized in 1% OG and incubated with increasing amounts of NADPH before the addition of 5 μM calmodulin. Cross-linking with EDC was performed as before. Increasing amounts of NADPH abolished the cross-link between Tic32 and calmodulin (CaM/Tic32 complex). (B) The interaction of Tic110 with Tic62 and Tic32 was analyzed by coimmunoprecipitation in the absence or presence of NADPH, NADP, or NADH. Coimmunoprecipitation was nearly abolished in the presence of NADPH but not affected by either NADP or NADH.