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Screening of RFamide derivatives on hNPFF2R. (A) hNPFF2R membranes were labeled with [125I]Tyr-NPFF, and three concentrations of RFamide derivatives were tested in competition experiments. Each concentration was tested in duplicate. Results for the reference and the six most active compounds are shown. Arrowheads indicate compounds that were selected for further characterization. (B) Structures of RF2, RF9, RF48, RF49, and BIBP3226.
