. Author manuscript; available in PMC: 2006 Nov 8.

Published in final edited form as: FASEB J. 2006 Jan;20(1):43–49. doi: 10.1096/fj.05-4707com

Table 1.

Maternal choline deficiency decreased DNA global methylation and increased expression of p15^INK4b, Kap and calretinin in E17 fetal hippocampus.

	Ammon's horn ventricularand subventricular zones		Dentate gyrus
	Control	Choline deficient	Control	Choline deficient
Global DNA	159.13 ± 6.7^a	109.9 ± 13.3^b	127.7 ± 20.3^a	117.2 ± 12.4^a
methylation	159.13 ± 6.7^a	109.9 ± 13.3^b	127.7 ± 20.3^a	117.2 ± 12.4^a
p15^INK4b	166.1 ± 7.9^a	220.9 ± 14.9^b	151.1 ± 32.5^a	171.1 ± 29.2^a
KAP	137.8 ± 16.2^a	290.3 ± 25.4^b	114.3 ± 11.3^a	134.1 ± 27.2^a
Calretinin	ND	ND	104.02 ± 4.34^a	195.55 ± 5.55^b

Pregnant mice were treated and fetal brains prepared as described in Figure 2 legend. DNA methylation and protein expression was assessed using immunohistochemical methods described in the Methods section. Optical density (OD) was measured by image analysis in selected areas of hippocampus: Ammon's horn ventricular and subventricular zones and the prime germinal zone of the dentate gyrus (for localization, see Figure 1). The computed values were divided by the number of cells in the selected areas. Results are expressed as OD mean values ± standard error (SE) of choline deficient and control samples. ND = not detected.

Values with different superscript letters are significantly different (p<0.05, Students t-test) within each assay.