Skip to main content
. 2006 Nov 8;103(47):17903–17908. doi: 10.1073/pnas.0608678103

Fig. 5.

Fig. 5.

mAb and C1 peptide binding to heat-triggered F-GCNt conformations. (A) Uncleaved or trypsin-cleaved F-GCNt was incubated at 25°C or 50°C for 30 min. Two samples were heated before trypsin cleavage (c, lanes 5 and 10). F-GCNt was immunoprecipitated with mAbs F1a or 6-7 and analyzed by reducing SDS/PAGE and immunoblot. (B) Uncleaved or trypsin-cleaved F-GCNt was incubated with HA-tagged C1 peptide for 30 min. Samples were heated to 50°C for 30 min at different points either during incubation with the C1 peptide (a), before adding peptide (b), or before trypsin cleavage (c). Samples in lanes 8–11 represent controls lacking C1 peptide. Peptide was immunoprecipitated by using 12CA5 mAb. The samples were analyzed by nonreducing SDS/PAGE and immunoblot.