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SD-MAPCs cultured in serum-free conditions with TGF-β1, PDGF-BB, TGF-β1 plus PDGF-BB, or without cytokine (Control). Expression is reported as percent expression compared with RAOSMCs on days 0, 2, 4, and 6 for α-SMA (A), SM22α (B), SM-MHC (C), calponin-1 (D), or myocardin (E). Expression on day 6 of all genes in the presence of TGF-β1 was significantly greater than in undifferentiated SD-MAPCs (day 0) (P < 0.01; n = 3–6).
