TABLE 3.
Impact of expressing cyp51 genes from Rhizopus oryzae on susceptibility of Saccharomyces cerevisiae YKKB-13 to antifungal agents
| Expressed gene | MIC (μg/ml)a
|
|||||
|---|---|---|---|---|---|---|
| Posaconazole | Itraconazole | Voriconazole | Fluconazole | Caspofungin | Amphotericin B | |
| Vector alone | 0.5 | 0.25 | 0.063 | 8 | 0.125 | 2 |
| S. cerevisiae ERG11 | 1 | 0.5 | 0.5 | 64 | 0.125 | 2 |
| R. oryzae cyp51A | 2 | 4 | >16 | >256 | 0.125 | 2 |
| R. oryzae cyp51B | 0.5 | 0.25 | 0.25 | 16 | 0.125 | 2 |
a
Triplicate independent MIC determinations were performed with yeast nitrogen base-leucine supplemented with 2% galactose and 1% raffinose and read at 72 h.