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HPLC and MALDI spectra for G10KHc. The quality of purified G10KHc was assessed by HPLC (A) and MALDI mass spectrometry (B). By monitoring the UV absorbance at 215 nm, a single peak that had the correct mass for G10KHc (4,267.44) was detected during HPLC (at 10.06 ml). mAU, milli-absorbance units.
