FIG. 5.

Dye uptake mediated by subinhibitory concentrations of G10KHc. P. aeruginosa cells were treated with medium (left column) or 2 μM G10KHc (right column) for 5 min, followed by PI dye addition. Bright-field (upper panels) and fluorescence (lower panels) images of the same field were collected and evaluated for intracellular dye accumulation (red fluorescence). The surviving numbers of CFU/ml from untreated (dye only) and G10KHc-treated cultures were quantitated after visualization and were plated as fivefold serial dilutions.