TABLE 2.

Ergosterol contents, p values, and passive diffusion of R6G in untreated and BPS- and BCS-treated wild-type cells and mutant cells with defective iron uptake

Strain (condition for growth)	% Ergosterola	p valueb	OD527c
CAI4	1.22 ± 0.05	0.171 ± 0.001	0.309 ± 0.023
CAI4 (200 μM BPS)	0.81 ± 0.02	0.08 ± 0.01	0.209 ± 0.02
CAI4 (500 μM BCS)	1.08 ± 0.04	0.113 ± 0.008	0.202 ± 0.033
Δftr1	0.97 ± 0.02	0.103 ± 0.01	0.237 ± 0.019
Δccc2	1.03 ± 0.033	0.114 ± 0.01	0.202 ± 0.02

^aThe mean ergosterol content of cells is expressed as a percentage of the wet weight of cells ± the standard deviation of three sets of experiments (33).

^bThe mean p value of cells (inversely proportional to membrane fluidity) ± the standard deviation of three sets of experiments is shown (32, 33, 35).

^cThe mean OD₅₂₇ of supernatant, for checking passive diffusion of R6G inside cells (a lower OD implies a higher concentration of R6G inside the cells), ± the standard deviation of three sets of experiments is shown (32, 33, 35).