FIG. 4.
Live-dead staining of C. albicans with fluorescein diacetate. Planktonic or biofilm cells were stained with 100 μg/ml fluorescein diacetate and examined by fluorescence microscopy. (A) Live planktonic cells. (B) Dead planktonic cells after treatment with 100 μg/ml amphotericin B (×400 magnification). (C, D, and E) Biofilms (×1,000 magnification) of untreated control and after 18 and 48 h of amphotericin B treatment (100 μg/ml), respectively.