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. Author manuscript; available in PMC: 2006 Nov 8.
Published in final edited form as: Tissue Eng. 2005;11(1-2):76–89. doi: 10.1089/ten.2005.11.76

FIG. 2.

FIG. 2.

Dot-blot analyses of FGF-2 binding to PlnDI. (A) Undigested PlnDI, and PlnDI digested with heparinases I, II, and III (HEPN) or chondroitinase AC (CHON), were blotted in triplicate on nitrocellulose membrane. (B) PBS, HEPN, and CHON only served as negative controls. FGF-2 binding was determined as described in Materials and Methods. (C) Bar graph summarizes densitometric measurements performed on dot blot (A) above. Each bar indicates the mean ± SD (n = 3).