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. Author manuscript; available in PMC: 2006 Nov 8.
Published in final edited form as: Tissue Eng. 2005;11(1-2):76–89. doi: 10.1089/ten.2005.11.76

FIG. 3.

FIG. 3.

(A) PlnDI binding to collagen I fibrils. Binding of biotinylated PlnDI was determined as described in Materials and Methods. The bar graph indicates means ± SD of triplicate determinations of PlnDI, PlnDI digested with heparinases I, II, and III (HEPN), and PlnDI digested with chondroitinase AC (CHON) to collagen I fibrils. Collagen I fibrils incubated in PBS without binding interaction with PlnDI served as control. *p ± 0.01 relative to undigested or chondroitinase-digested PlnDI. (B) FGF-2 binding to PlnDI associated with collagen I fibrils. PlnDI and PlnDI digested with heparinases I, II, and III (HEPN) interact with collagen I fibrils to form PF (PlnDI-fibrils) and D-PF (digested PlnDI-fibrils), respectively. These complexes were used as matrices for FGF-2 binding. FGF-2 binding was determined by ELISA as described in Materials and Methods. Each bar indicates the mean ± SD of triplicate determinations.