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. 2006 Sep 13;34(17):4743–4751. doi: 10.1093/nar/gkl553

Figure 2.

Figure 2

Substrate specificity of Endo IV. The activity of Endo IV (1, 0.5, 0.2, 0.1 or 0.05 μg/ml) was assayed with phiX174 RFI circular dsDNA (A), phiX174 RFI linear dsDNA (B), phiX174 virion circular ssDNA (C), linear ssRNA used for the in vitro synthesis of the GST-Endo IV fusion protein (D), heat-denatured T4 genomic dsDNA containing gluc-dHMC (E), or heat-denatured dC-substituted T4 (T4dC) genomic dsDNA (F) as the substrate (5 μg/ml). The reaction products were separated by electrophoresis on a 1.0% agarose gel and stained with SYBR Gold. Lanes M and (–) contain a 1 kb DNA ladder and a reaction mixture incubated in the absence of the enzyme, respectively.