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Gel mobility shift assay of KGZIP with a Z-DNA substrate. KGZIP was incubated with a Z-DNA substrate, d(G-5BrC)20 labeled with 32P, (open arrow) in absence of B-DNA competitor. Decreasing concentrations of KGZIP (from 1 μM to 7.8 nM, in 2-fold serial dilutions) were incubated with the probe. A control lane (−) without KGZIP is shown in the same gel. KGZIP:Z-DNA complexes are indicated by solid arrows.
